{"id":9726,"date":"2018-11-16T09:40:30","date_gmt":"2018-11-16T14:40:30","guid":{"rendered":"https:\/\/biology.mit.edu\/?p=9726"},"modified":"2018-11-16T09:40:30","modified_gmt":"2018-11-16T14:40:30","slug":"activating-a-new-understanding-of-gene-regulation","status":"publish","type":"post","link":"https:\/\/biology.mit.edu\/activating-a-new-understanding-of-gene-regulation\/","title":{"rendered":"Activating a new understanding of gene regulation"},"content":{"rendered":"

CAMBRIDGE, Mass. – Regulation of gene expression \u2014 turning genes on or off, increasing or decreasing their expression \u2014 is critical for defining cell identity during development and coordinating cellular activity throughout the cell\u2019s lifetime. The common model of gene regulation imagines the nucleus of the cell as a large space in which molecules involved in DNA transcription float around seemingly at random until they stumble across a DNA sequence or other transcriptional machinery to which they can bind \u2014 a haphazard approach. However, this paradigm is being upended as over the last few years researchers have discovered that rather than being amorphous spaces dependent upon fortuitous collisions, cells actually compartmentalize their processes into discrete membraneless structures in order to congregate relevant molecules, thereby better coordinating their interactions. Research from the lab of Whitehead Member\u00a0Richard Young<\/a>\u00a0and others earlier this year reported that such compartmentalization is a crucial, previously unobserved aspect of gene regulation.[1]<\/p>\n

The latest research from Young\u2019s lab, published online November 15 in the journal\u00a0Cell<\/em><\/i>, delves further into how such compartmentalization helps orchestrate transcriptional regulation by revealing the role of the activation domain, a part of transcription factors previously shrouded in mystery. One side of transcription factors, containing the DNA binding domain, binds to a region of DNA near a gene. The other end, called the activation domain, then captures molecules that impact gene expression, anchoring that transcriptional machinery near the gene.<\/p>\n

This most recent work reveals that activation domains do their job by meshing with other transcription proteins to form liquid droplets near the genes they regulate. The process by which the molecules form a distinct liquid compartment within the environment of the cell \u2014 like oil refusing to mix with vinegar in a salad dressing \u2014 is called phase separation.<\/p>\n

Such an evolved understanding of gene regulation has enormous implications for medicine and drug discovery, as errors in gene regulation are key components of many diseases, including cancers. The new model could help illuminate how diseases coopt regulatory mechanisms and how therapeutic interventions might remedy such dysregulation. Transcription factors have traditionally been hard to target therapeutically, and the incomplete understanding of their structure and function may have been part of the reason.<\/p>\n

\u201cTranscriptional regulation is important for every human function, from cell differentiation to development to cell maintenance,\u201d says Ann Boija, co-first author and postdoctoral researcher in Young\u2019s lab. \u201cDespite that fact the structure and function of the activation domain on the transcription factors have been poorly understood.\u201d<\/p>\n

Most proteins settle into defined three-dimensional structures and can only bind with other molecules that fit them perfectly, in a specific orientation, like a key in a lock. The activation domains of transcription factor proteins, however, contain what are known as intrinsically disordered regions, which behave more like strands of cooked spaghetti, tangling at random into flexible shapes. This disorder allows the molecules to bind at many points, creating a dynamic network of loose connections that appears to precipitate phase separation.<\/p>\n

\u201cI have taught regulatory biology for decades using inspiration from lock and key structures. They are elegant, and easy to visualize and model, but they don\u2019t tell the whole story. Phase separation was the missing piece,\u201d says Young, who is also a professor of biology at MIT.<\/p>\n

In experiments with a variety of transcription factors, Boija and co-first author Isaac Klein, a postdoctoral researcher in Young\u2019s lab and medical oncology fellow at the Dana-Farber Cancer Institute, found that the transcription factors meshed with Mediator, a molecule that helps activate genes, and phase separated into droplets, and that this process was associated with gene activation. The transcription factors they investigated included OCT4, which is important for maintaining the state of embryonic stem cells; the estrogen receptor (ER), which plays a role in breast cancer; and GCN4, a well-studied model transcription factor in yeast.<\/p>\n

The discovery has implications for many diseases, such as cancer, in which cancer genes may use phase separated droplets to help ramp up their expression. New therapeutic approaches could focus on dissolving the droplets, and drug discovery can incorporate testing of how the drug \u2014 or target molecule \u2014 behaves inside versus outside of the droplets. This new model of how transcription factors function is not only rewriting the understanding of transcriptional regulation, it is opening up new paths for drug discovery and therapeutic approaches.\u201cWe found a link between gene activation and phase separation across a broad spectrum of contexts,\u201d Klein says, suggesting that this mechanism is a common feature of transcriptional regulation.<\/p>\n

The work was supported by the National Institutes of Health (NIH grants GM123511, GM117370, T32CA009172, T32GM08759), the National Science Foundation (NSF grant PHY1743900), Swedish Research Council (VR 2017-00372), Damon Runyon Cancer Research Foundation (2309-17), Hope Funds for Cancer Research, Cancer Research Institute, and Netherlands Organisation for Scientific Research (NWO).<\/p>\n

***<\/h5>\n
Richard Young\u2019s primary affiliation is with Whitehead Institute for Biomedical Research, where his laboratory is located and all his research is conducted. He is also a professor of biology at Massachusetts Institute of Technology.<\/h5>\n
***<\/h5>\n
Full citation:<\/h5>\n
\u201cTranscription factors activate genes through the phase separation capacity of their activation domains\u201d<\/h5>\n
Cell<\/em><\/i>, online November 15, DOI: 10.1016\/j.cell.2018.10.042<\/h5>\n
Ann Boija (1,7), Isaac A. Klein (1,2,7), Benjamin R. Sabari (1), Alessandra Dall’Agnese (1), Eliot L. Coffey (1,3), Alicia V. Zamudio (1,3), Charles H. Li (1), Krishna Shrinivas (4,5), John C. Manteiga (1,3), Nancy M. Hannett (1), Brian J. Abraham (1), Lena K. Afeyan (1,3), Yang E. Guo (1), Jenna K. Rimel (6), Charli B. Fant (6), Jurian Schuijers (1), Tong Ihn Lee (1), Dylan J. Taatjes (6), and Richard A. Young (1,3)<\/h5>\n
    \n
  1. \n
    Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA<\/h5>\n<\/li>\n
  2. \n
    Department of Medical Oncology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02215, USA<\/h5>\n<\/li>\n
  3. \n
    Department of Biology<\/h5>\n<\/li>\n
  4. \n
    Department of Chemical Engineering<\/h5>\n<\/li>\n
  5. \n
    Institute of Medical Engineering and Science, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA<\/h5>\n<\/li>\n
  6. \n
    Department of Biochemistry, University of Colorado, Boulder, CO 80303, USA<\/h5>\n<\/li>\n
  7. \n
    These authors contributed equally<\/h5>\n<\/li>\n<\/ol>\n
    [1] Sabari et al., \u201cCoactivator condensation at super-enhancers links phase separation and gene control,\u201d<\/h5>\n
    Science<\/em>, June 21, 2018; Cho et al., \u201cMediator and RNA polymerase II clusters associate in transcription-dependent condensates,\u201d\u00a0Science<\/em>, June 21 2018.<\/h5>\n","protected":false},"excerpt":{"rendered":"

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